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不同植物生长调节剂对草莓组培苗快繁的影响

作者:组培架 发布时间:2020-07-07 18:24 浏览次数:

文章来源:组培架

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  草莓是蔷薇科草莓属多年生草本植物,在云南省栽培面积逐步扩大,目前草莓生产栽培上主要为匍匐茎繁殖和分株繁殖。这种繁殖方法效率低、种苗易退化,同时易造成病毒蔓延,严重影响草莓的长势、品质和产量。通过草莓组织培养扩繁草莓脱毒苗,可以解决上述问题。组织培养技术应用于草莓生产,能在较短时间内提供大量整齐一致的良种苗和脱毒苗。

组织培养

捕鱼大作战  BENSHIYANZHUYAOTONGGUODUIBIPEIYANGJIBUTONGJISUTIAOJIAN,TANTAOZHIWUSHENGZHANGDIAOJIEJITIAOJIANDUIJINGTAOXIANGCAOMEIJINGJIANPEIYANGXIAOGUODEYINGXIANG,YIWEIMANZUCAOMEIGUIMOHUASHENGCHANDIANDINGJICHU。

  1 CAILIAOYUFANGFA

  1.1 SHIYANCAILIAO

  BENYANJIUYIHONGHEZHOUJINGJIZUOWUJISHUTUIGUANGZHANDAPENGNEIJINGTAOXIANGCAOMEIMIAOWEISHIYANCAILIAO,QIEQUCAOMEIMIAOPUFUJINGJIANZUOWEIWAIZHITI。

  1.2 SHIYANFANGFA

捕鱼大作战  1.2.1 QUCAIHEXIAODU。JIANQUJINGTAOXIANGCAOMEIPUFUJINGJIAN,ZHANGYUE2 cm,JIANQUYEPIAN,YONGZILAISHUICHONGXIGANJING。ZAICHAOJINGGONGZUOTAISHANG,YONG70%JIUJINGXIAODU30sHOUYONGWUJUNSHUICHONGXI1CI,ZAIYONG0.1%LVHUAGONGMIEJUN15min,WUJUNSHUICHONGXI3CI,RANHOUYONGJIEPOUZHENBOQUJINGJIANWAIMIANDEYOUYEHELINPIAN,LUCHUSHENGZHANGDIAN,DAIJIEZHONG。

  1.2.2 BUTONGZHIWUSHENGZHANGDIAOJIEJICHULISHEJIHEPEIYANG。GONGSHE5GECHULI,FENBIEWEICHULI 1:MS+6-BA 0.5 mg/L+NAA 0.2 mg/L;CHULI 2:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L;CHULI 3:MS+6-BA0.4 mg/L+NAA 0.1 mg/L;CHULI 4:MS+6-BA 1.0 mg/L;CHULI 5:MS+6-BA 0.5 mg/L+GA30.2 mg/L。ZHETANG 30 g/L,QIONGZHI 7 g/L,pH ZHI 5.8~6.0。JIEZHONGSHIMEIPINGJIEZHONG1~2GEWAIZHITI,MEIGECHULI15PING,JIEZHONGHOUJIANGPINGKOUZHAHAO、BIAOHAO。PEIYANGTIAOJIANWEI:WENDU25~30℃,GUANGZHAOQIANGDU1 500~2 000 lx,MEITIANGUANGZHAO10 h,PEIYANG20 dHOUHUAN1CIPEIYANGJI,JIDAIPEIYANG3DAIHOUDIAOCHASHENGZHANGQINGKUANG。

捕鱼大作战  1.3 DIAOCHANEIRONGYUFANGFA

捕鱼大作战  TONGJIZUPEIMIAOXIANZHONG、ZHUGAO,MEIZHUMIAOFENHUACHUDEYOUMIAOSHUZUOWEIFANZHIXISHU。3CIZHONGFU,JINGQUECELIANGJISUANJUNZHI,GUANCHAZUPEIMIAODEZHANGSHI、HUANGHUAZHUANGKUANGDENG。

组织培养

  2 JIEGUOYUFENXI

  2.1 BUTONGCHULIDUIZENGZHIXISHUDEYINGXIANG

  SHIYANJIEGUOBIAOMING,BUTONGCHULIDUICAOMEIZUPEIMIAOFANZHIXISHUYOUYIDINGYINGXIANG,QIZHONGCHULI 4MINGXIANGAOYUCHULI 1、2、3、5,6-BA WEIXIBAOFENLIESUCUJINXIBAOFENLIE,6-BANONGDUYUEDA,FANZHIXISHUYUEGAO。

  2.2 BUTONGCHULIDUIXIANZHONGDEYINGXIANG

  CONGZHENGTISHANGKAN,CHULI 4DEXIANZHONGMINGXIANCHAYUCHULI 1、2、3、5,SHUOMING6-BANONGDUDUIXIANZHONGYINGXIANGJIAODA,6-BANONGDUZENGJIA,XIANZHONGJIANGDI。QIZHONG,0.5 mg/L 6-BAGENGYOULIYUZUPEIMIAOXIANZHONGDEZENGJIA。ZONGSHANGSUOSHU,YICHULI1(6-BA 0.5mg/L+NAA 0.2 mg/L)ZUISHIHEJINGTAOXIANGCAOMEIZUPEIMIAOXIANZHONGDEZENGJIA。

  2.3 BUTONGCHULIDUIZHUGAODEYINGXIANG

捕鱼大作战  CONGZHENGTISHANGKAN,CHULI1、2、3、5DEXIAOGUOHAOYUCHULI4,SHUOMING6-BANONGDUDABINGBUYOULIYUZHUGAODEZENGJIA,YI6-BA 0.5 mg/LYOULIYUZHUGAODEZENGJIA。

  2.4 BUTONGCHULIDUIZUPEIMIAOZHANGSHIDEYINGXIANG

捕鱼大作战  CONGZHENGTISHANGKAN,6-BANONGDUGUOGAO、BUTIANJIASHENGZHANGSUNAA,BULIYUCAOMEIZUPEIMIAODESHENGZHANG,ZHANGSHIRUO,BINGHUICHUXIANHUANGHUAMIAO。

  3 JIELUNYUTAOLUN

  3.1 JIELUN

  SHIYANJIEGUOBIAOMING,6-BANONGDUDUIZUPEIMIAODEFANZHIXISHUYINGXIANGBIJIAOMINGXIAN,6-BANONGDUGAOSHICAOMEIZUPEIMIAOFANZHIXISHUMINGXIANZENGJIA。BUTONGJISUTIAOJIANDUICAOMEIZUPEIMIAOXIANZHONGYINGXIANGHENDA,GAONONGDUDE6-BAHUIYIZHICAOMEIMIAOXIANZHONGDEZENGJIA,YIZHICAOMEIMIAOSHENGZHANG,ZHUGAOJIANGDI,ZHANGSHIRUO,BINGBANYOUHUANGHUAMIAOCHUXIAN。TONGGUOSHIYANDUIBIKEDE,JINGTAOXIANGCAOMEIZUPEIFANZHIYI6-BANONGDUWEI0.5mg/LWEISHIYI,SHENGZHANGSUNAA0.2mg/LJIAO0.1 mg/LSHAOYOULIYUCAOMEIXIANZHONGJIZHUGAODEZENGJIA,DANSHIZENGZHIXISHUDI,ZAIJIDAIPEIYANGZHONGCAOMEIMIAORONGYIFAGEN,BINGHUICHUXIANKAIHUAXIANXIANG。ZONGHEDUIBI,JINGTAOXIANGCAOMEIZUPEIMIAOKUAIFANDEZUIJIAZUPEITIAOJIANWEIMS+6-BA 0.5 mg/L+NAA 0.1 mg/L。

组织培养

  3.2 TAOLUN

捕鱼大作战  YANJIUBIAOMING,JISUSHIYINGXIANGZUPEIMIAOSHENGZHANGDEYIGEGUANJIANYINSU,YOUDAOFENSHENGMIAOZENGZHI、CUJINZUPEIMIAOSHENGZHANGHUOYOUDAOGEN,SUOXUZHIWUJISUDEZHONGLEIHENONGDUZAIBUTONGPEIYANGJIEDUANSHIBUTONGDE。ZUPEIGUOCHENGZHONG,SHENGZHANGSUZHUYAOBEIYONGZUOYOUDAOCIJIXIBAOFENLIEHEGENDEFENHUA。ZAITIAOJIANXIANGTONGDEQINGKUANGXIA,SHIYONG6-BADEZHIZHUZHENGTIZHANGSHIYAOHAOYUSHIYONGNAA,DANNONGDUDADAO1.0 mg/LSHI,SUIRANFANZHIXISHUJIAODA,DANTONGSHIYEYIZHILEMIAODESHENGZHANG。BENYANJIUBIAOMING,DANGXIBAOFENLIESUYUSHENGZHANGSUBIZHIDISHICUJINGENDEFASHENG,DANGBIZHIGAOSHILIYUYADEFASHENG;6-BAHENAANONGDUDEBIANHUADOUHUIYINQIZUPEIMIAOZHONGLIANGDEBIANHUA;NAAZAIZENGJIAZHUGAOFANGMIANDEXIAOGUOHAOYU6-BA。

捕鱼大作战  CAOMEIZUPEIMIAOSHENGGENRONGYI,JISHIMEIYOUSHENGGENDECAOMEIZUPEIMIAOXUNHUACHENGHUOLVYEZAI80%YISHANG,YINERBENSHIYANWEIDUIJINGTAOXIANGCAOMEIDESHENGGENJINXINGGUODUOYANJIU,DANSHIZAIBUTIANJIARENHESHENGZHANGJISUDEMSPEIYANGJISHANG,JINGTAOXIANGCAOMEIYENENGHENHAODISHENGZHANG,FAGENDUOQIEZHANG。

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