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鸟巢蕨组培瓶苗培育技术

作者:腾昊组培室 发布时间:2019-02-27 10:44 浏览次数:

文章来源:腾昊组培室

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  DUINIAOCHAOJUEAsplenium nidusYOULIANGZHUXIBAOZILITIPEIYANGDECHUDAIPEIYANG、JIDAIPEIYANGHESHENGGENPEIYANGYIJIYIZAIDENGFANGMIANJINXINGYANJIU。JIEGUOBIAOMING,CHUDAIPEIYANGDEJIAPEIYANGJISHIMS+0.1%HUOXINGTAN,YUANYETIXINGCHENGLVDA80%;ZAI1/3MS+6-BA 1.8 mg·L-1+NAA 0.5 mg·L-1ZENGZHIPEIYANGJIZHONGPEIYANG,ZENGZHIBEISHUGAODA5.1;JIDAIYAMIAOZAI1/2MS+NAA 0.5 mg·L-1+IBA 0.5 mg·L-1SHENGGENPEIYANGJIZHONG,SHENGGENLVGAODA95%;YIZAIDEJIAJIZHIWEIHONGXINTU+FUZHITU(1∶1),YIZAICHENGHUOLVDA90%。

捕鱼大作战  NIAOCHAOJUEAsplenium nidusSHUYUTIEJIAOJUEKEAspleniaceaeCHAOJUESHU,YOUMINGSHANSUHUA、QUECHAOJUE、QUECHAOYANGCHI,CHANYUYAZHOU、FEIZHOUHEAOZHOU。NIAOCHAOJUECHANGYONGZUOPENHUAJIJIATINGYUANYIZAOJINGCAILIAO。JINNIANLAI,QIYINNENYEXIANLV、CHAOSHIZHICUISHUANGKOU,BEIYONGZUOGAODANG、DAODIDEYUANSHENGSHUCAI。NIAOCHAOJUEMEIYOUPUFUJING,ZHUYAOKAOBAOZIFANZHI,CHANGGUIBOZHONGYISHOUZIRANTIAOJIANYINGXIANGZHIYOUDAOLVDI。MUQIANYIJINGYOUHENDUOJUELEIZHIWUZUZHIPEIYANGJIWUXINGXIJIANLIDEBAODAO,DANGUONEIDUINIAOCHAOJUEDEYANJIUZHUYAOJIZHONGZAIBAOZIWUJUNBOZHONG、NENYEFANZHIDENGZUPEIFANZHIJISHU,QUDELEYIDINGCHENGGUO,DANHAIBUNENGMANZUNIAOCHAOJUEGUIMOHUASHENGCHANDEXUYAO。YINCI,BENYANJIUYINIAOCHAOJUEBAOZIWEIWAIZHITI,DUINIAOCHAOJUEJINXINGZUZHIPEIYANGYUMIAOSHIYAN,ZHIZAIWEINIAOCHAOJUEDESHANGYEHUAFANYUTIGONGLILUNHEJISHUZHICHI。

  1 CAILIAOYUFANGFA

  1.1 CAILIAO

捕鱼大作战  ZAIFUJIANSHENGLINYEKEJISHIYANZHONGXINJUELEIPEIYUJIDIXUANQUZHANGSHIWANGSHENGDE2~3NIANSHENGNIAOCHAOJUEYOULIANGDANZHUDEBAOZIZUOWEIWAIZHITI。

  1.2 FANGFA

捕鱼大作战  1.2.1 WAIZHITIXIAODUYONGSHABUSHOUJINIAOCHAOJUEYEPIANSHANGDEBAOZIBINGBAOZHAHAO,ZILAISHUICHONGXI1~1.5 h;ZAICHAOJINGGONGZUOTAISHANGYONG70%JIUJINGXIAODU30 s,ZAIYONG0.1%SHENGGONGMIEJUN5 min,WUJUNSHUICHONGXI3CI。

  1.2.2 BAOZIMENGFAPEIYANGJIANGXIAODUDEBAOZIJIERUJIBENPEIYANGJI,FUJIABUTONGNONGDUHUOXINGTAN(0、0.1%、0.3%)HOUZHIYUPEIYANGSHIPEIYANG。SHIYANCAIYONGDANYINSUSUIJIQUZUSHEJI[10],MEI5 dGUANCHA1CI,GUANCHAQISHIBAOZIMENGFASHIJIAN,TONGJIYUANYETIXINGCHENGLV。

  1.2.3 ZENGZHIYUFENHUAPEIYANGYOUDAOPEIYANGYUE40 dHOU,JIANGCHUDAIPEIYANGDEYUANYETITUANJINXINGJIDAIPEIYANGYUKUOFAN。PEIYANGJIGENJUQIDONGPEIYANGWAIZHITIDESHENGZHANGQINGKUANG,DIAOZHENGPEIYANGJICHENGFENJISHENGZHANGDIAOJIEJISHUIPING(PEIYANGJI、6-BA、NAA),YIQIDEDAOJIAPEIYANGXIAOGUO。SHIYANCAIYONGL9ZHENGJIAOSHEJI,YIKONGBAIZUODUIZHAO。MEIDAIPEIYANG25 d,LIANXUZENGZHI3DAI,TONGJIZENGZHIBEILV。

捕鱼大作战  1.2.4 SHENGGENPEIYANGTUANZHUANGTI、NIAOCHAOZHUANGXIAOMIAOZHANGZHI1.8 cmYISHANGSHI,FENCHENGDANMIAOZHUANRUBUTONGSHENGZHANGDIAOJIEJIZUHEDESHENGGENPEIYANGJIZHONGPEIYANG。SHENGGENPEIYANGJI:(1) 1/2MS+NAA 0.5 mg·L-1;(2) 1/2MS+NAA 0.5 mg·L-1+IBA 0.5 mg·L-1;(3) 1/2MS+IBA 0.5 mg·L-1。SHIYANCAIYONGDANYINSUSUIJIQUZUSHEJI,30 dGUANCHASHENGGENQINGKUANG。

  1.2.5 PEIYANGTIAOJIANCHUDAIPEIYANGJIHEZENGZHIPEIYANGJIJIAZHETANG30 g·L-1、QIONGZHI4.5 g·L-1;SHENGGENPEIYANGJIJIAZHETANG20 g·L-1、KALAJIAO6.2 g·L-1。MEICHULI50PING,MEIPING5ZHU。PEIYANGJIpH 5.8,PEIYANGWENDU23 ± 2 ℃,GUANGZHAOQIANGDU1500 lx,GUANGZHAOSHIJIAN12 h·d-1。

捕鱼大作战  1.2.6 YIZAIJIANGGAO2~4 cmSHENGGENMIAOLIANMIAO7 d,YICHUPEIYANGPING,XIJINGPEIYANGJI,YIZHIYUHONGXINTU+NITANTU(1∶1)、HONGXINTU+FUZHITU(1∶1)HEHONGXINTU+FUZHITU(1∶3)DENG3ZHONGJIZHI,YIZAIHOU5 dNEI,MIAOCHUANGGAIBOMOBAOSHI,JIAGAI85%HEISHAWANGZHEYIN。CAIYONGDANYINSUSUIJIQUZUSHEJI,3CIZHONGFU,MEIZHONGFU200DAI,YIZAIHOU30 dTONGJICUNHUOLV。

  2 JIEGUOYUFENXI

  2.1 HUOXINGTANDUIWAIZHITIBAOZIMENGFADEYINGXIANG

捕鱼大作战  NIAOCHAOJUEWAIZHITIBAOZIJINGGUO15~50 dPEIYANG,PEIYANGJIBIAOMIANKEJIANJIAODUOLVSEXIAODIAN,HUOLVDIANZENGDAXINGCHENGYUANYETI。PEIYANGGUOCHENGZHONG,HUOXINGTANDUINIAOCHAOJUEBAOZIMENGFAQICUJINZUOYONG。ZAIYIDINGNONGDUFANWEINEI,SUIZHEHUOXINGTANNONGDUDEZENGJIANIAOCHAOJUEBAOZIMENGFASHIJIANSUODUAN、YUANYETIXINGCHENGZENGDUO。ZAI0.1%HUOXINGTANSHI,BAOZIMENGFASHIJIANDUAN,WEI35 d,TONGSHIYUANYETIXINGCHENGLVGAO,WEI80%(BIAO1)。YINCI,NIAOCHAOJUEBAOZIMENGFAJIAPEIYANGJISHIMS+0.1%HUOXINGTAN。

  2.2 BUTONGPEIYANGJIJISHENGZHANGDIAOJIEJIZUHEDUIYUANYETIZENGZHI、FENHUAPEIYANGDEYINGXIANG

  CHUDAIPEIYANGYUANYETITUANZAIZENGZHIPEIYANGJIZHONG,XIANCONGJIBUKAISHICHANSHENGBUGUIZEDETUQI,ERHOUZHANGCHENGLVSEQIUZHUANGTI,BINGBUDUANZENGZHIXINGCHENGTUANZHUANGTI。PEIYANG25 dZUOYOU,YUANYETITUANZHONGYOUXIAOMIAOFENHUAXINGCHENG。JIEGUOBIAOMING,6-BADUIZUPEIZENGZHIPEIYANGDEYINGXIANGDAXIANZHUSHUIPING,QIEZENGZHIBEISHUSUIZHE6-BANONGDUTIGAOZHUJIANZENGJIA,YI6-BA 1.8 mg·L-1WEIHAO;ERJIBENPEIYANGJIHENAAFENBIEYIB3、C2SHUIPINGWEIHAO(BIAO2)。9ZHONGZUHESHIYANZHONG,BUTONGCHULIZUHEJIANDEZENGZHIBEISHUCUNZAIXIANZHUCHAYI,YI1/3MS+6-BA 1.8 mg·L-1+NAA 0.5 mg·L-1ZUHEWEIHAO,ZENGZHIXISHUGAODA5.1。

  2.3 SHENGZHANGDIAOJIEJIDUITUANZHUANGTISHENGGENPEIYANGDEYINGXIANG

  TUANZHUANGTI、NIAOCHAOZHUANGXIAOMIAOPEIYANG30 d,ZAIMIAOJIBUXINGCHENGZONGHESEGEN,GENSHANGYOUGENMAOHECHAGEN。JIEGUOBIAOMING,PEIYANGJI(1)、(3)ZHONGXINGCHENGDEGENTIAOSHUSHAO;PEIYANGJI(2)NENGCHANSHENG3TIAOYISHANGDEGEN,GENZHANG2~4 cmBUDENG,SHENGGENLVDA95%YISHANG(BIAO3)。YINCI,SHENGGENPEIYANGJIYIPEIYANGJI(2)(1/2MS+NAA 0.5 mg·L-1+IBA 0.5 mg·L-1)WEIHAO。

捕鱼大作战  BIAO1 HUOXINGTANDUIBAOZIMENGFADEYINGXIANG

捕鱼大作战  Table 1 Effects of activated carbon on spore germination

  


捕鱼大作战  BIAO2 BUTONGPEIYANGJIJISHENGZHANGDIAOJIEJIZUHEDUIYUANYETIZENGZHIPEIYANGDEYINGXIANG

  Table 2 Effects of different combinations of medium and growth regulators on multiplication culture of prothallus

  


  2.4 BUTONGDEYIZAIJIZHIDUIYIZAICHENGHUOLVDEYINGXIANG

  JIZHIDUIYIZAICHENGHUOLVWUXIANZHUYINGXIANG。YIZAICHENGHUOLVDOUHENGAO,DANYIHONGXINTU+FUZHITU(1∶1)PEIBIDEJIZHIWEIHAO,DA90%(BIAO4)。

  组培瓶苗移出培养瓶之前,要在温室大棚放置(炼苗)7 d,提高抗逆性。将组培生根苗,移入各种经消毒后的基质,移栽后5 d内,苗床盖上薄膜保湿,加盖85%黑纱网,避免阳光直射。

  BIAO3 BUTONGSHENGZHANGDIAOJIEJIDUISHENGGENPEIYANGDEYINGXIANG

  Table 3 Effects of different growth regulators on rooting culture

  


  ZHU:PEIYANGJI(1)1/2MS + NAA 0.5 mg·L-1;(2) 1/2MS + NAA 0.5 mg·L-1+ IBA 0.5 mg·L-1;(3) 1/2MS + IBA 0.5 mg·L-1。

  BIAO4 BUTONGYIZAIJIZHIDUISHENGGENMIAOYIZAIDEYINGXIANG

捕鱼大作战  Table 4 Effects of different transplant matrix on transplanting of rooting seedlings

  


  3 JIELUN

捕鱼大作战  ZAIBENSHIYANZHONG,YINIAOCHAOJUEBAOZIWEIWAIZHITI,JIAYOUDAOPEIYANGJIWEIMS+0.1%HUOXINGTAN,BAOZIMENGFASHIJIANDUAN,WEI35 d,YUANYETIXINGCHENGDUO,WEI80%;ZENGZHIPEIYANGJIYOUSHUIPINGZUHEWEI1/3MS+6-BA 1.8 mg·L-1+NAA 0.5 mg·L-1,ZENGZHIXISHUGAODA5.1;JIASHENGGENPEIYANGJIWEI1/2MS+NAA 0.5 mg·L-1+IBA 0.5 mg·L-1,SHENGGENLV95%YISHANG,GENXICUZHUANG,GENSHUDUO。DUIYIZAISUOXUANJIZHIDUIYIZAICHENGHUOLVWUXIANZHUYINGXIANG,DANSHIYIHONGXINTU+FUZHITU(1∶1)PEIBIDEJIZHIWEIHAO,DA90%。

捕鱼大作战  WAIZHITIYOUDAOSHIYANBIAOMING,CAIYONGWUHUOXINGTANDEPEIYANGJIYOUDAOBAOZIMENGFASHIJIANZHANG、YUANYETIXINGCHENGLVDI,BULIYUCHANYEHUASHENGCHAN。YINCI,CAIYONGHANHUOXINGTANDEPEIYANGJIYOUDAOWAIZHITI,YOULIYUNIAOCHAOJUEGUIMOHUASHENGCHAN

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