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生物消毒剂在植物组培中的应用

作者:组培设备 发布时间:2020-04-13 18:30 浏览次数:

文章来源:组培设备

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  1 KANGSHENGSU

  抗生素可干扰病原微生物的代谢过程,如抑制细菌细胞壁的合成、影响细菌细胞膜的通透性、抑制菌体蛋白质的合成、抑制细菌核酸的合成等,从而起到抑菌或杀菌作用.利用不同浓度、不同种类的抗生素做抑菌实验的结果表明,在同等浓度下庆大霉素对细菌产生了明显的抑制作用,青霉素与氯霉素也能抑制细菌的生长,但抑菌效果不如庆大霉素,四环素明显的抑菌作用.随后,很多学者在植物组培的无菌处理环节上使用了抗生素.

植物组培

捕鱼大作战  1.1 KANGSHENGSUZAIWAIZHITIYUCHULIZHONGDEYINGYONG

捕鱼大作战  JIANGDENGLIANGWAIZHITIFENBIEJINPAOZAIHAN400mg/LANBIANQINGMEISUHE200mg/LQINGDAMEISUDERONGYEZHONG1h,FAXIANJINGKANGSHENGSUCHULIGUODEWAIZHITIKAISHISIWANG.ZAIYANJIUKONGZHISHENGJIANGZUZHIPEIYANGWURANLVDEGUOCHENGZHONGFAXIAN,YONGLIUSUANKANEIMEISUHELIUSUANQINGDAMEISURONGYEJINPAOWAIZHITINENGYOUXIAOKONGZHIWURAN,TONGSHIBUHUIYIZHIYADESHENGZHANG,DANYONGTOUBAOANBIANDEXIAOGUOCIZHI,QINGMEISUDEYIJUNXIAOGUOBULIXIANG.YANJIUFAXIAN,1g/L DUOJUNLING、800mg/L SUOBIANQINGMEISUNAHE500mg/LSAIBAOMEISUHUNHEHOUCHULIWAIZHITI,ZHENJUNHEXIJUNWURANLVDOUXIANZHUXIAJIANG.YISHANGDEYINGYONGSHUOMING,LIYONGKANGSHENGSUYUCHULIWAIZHITISHIBIXUSHAIXUANHESHIDEZHONGLEI、ZHANGWOQIADANGDENONGDUHECHULISHIJIAN.

  1.2 PEIYANGJIZHONGTIANJIAKANGSHENGSU

  KANGSHENGSURONGYECHULEYONGYUWAIZHITIDEXIAODUMIEJUN,HAIKEJIARUDAOPEIYANGJIZHONGQIDAOCHIXUYIJUNDEZUOYONG,CONGERJIANSHAOZUPEIZHONGDEWURAN,YOUQIDUIZHIWUNEISHENGJUNDEYIZHIYOUMINGXIANDEZUOYONG.MUQIAN,SHIYONGDANYIKANGSHENGSUJINXINGYIJUNDEYANJIUJIAODUO,YONGZHUSHEQIZAIDENGENANFENHUAPEIYANGJIHESHENGGENPEIYANGJIZHONGJIARUBUTONGNONGDUDEBUTONGKANGSHENGSU,FAXIANFUJIAANBIANXILINNA100mg/L,DUIDANGDAIWURANJUNDEYIJUNLVWEI100%,TONGSHIZAIZENGZHIXISHU、MIAOGAO、SHENGZHANGSHI、SHIGUANMIAOSHENGGENDENGFANGMIANYEDOUBIAOXIANZUIHAO,LIUSUANLIANMEISUKANGJUNXIAOGUOCIZHIDANDUISHIGUANMIAOCHANSHENGDUHAI,QINGDAMEISUKANGJUNXIAOGUOBUJIA.ZAIYINJINDE2GEPUTAOPINZHONGWEIDEHENANFANGWUHEDEZUPEIZHONG,ZAIQICHUDAIPEIYANGJIZHONGFENBIEJIARUBUTONGNONGDUDEQINGMEISU20mg/LHE40mg/L、SIHUANSU20mg/LHE40mg/LLAIYIZHIZHENJUNHEXIJUNDESHENGZHANG,JIEGUOBIAOMINGSIHUANSUDEYIJUNZUOYONGBIQINGMEISUDEGENGHAO,LIANGZHONGKANGSHENGSUZAISHIYANNONGDUXIADOUMEIYOUDUIZHIWUTICHANSHENGDUHAIZUOYONG,KEYINGYONGYUSHIJISHENGCHAN.YINVWANGZHUYUFENNIEYAWEICAILIAO,FAXIANZAIPEIYANGJIZHONGTIANJIAQINGDAMEISUKEYIZHIGONGSHENGJUNZAOCHENGDEWURAN,QIZUIJIANONGDUWEI60mg/L.ZAIZAOZUZHIPEIYANGZHONGFAXIANKESHIYONG150mg/LTOUBAOMEISULAIYIZHIWURAN.ZAIBAIWANGWENCAOZUZHIPEIYANGZHONGJIARU20~30mg/LDETUMEISU,WURANLVJIANGDI25GEBAIFENDIANYISHANG.

  CAIYONGYIYONGKANGSHENGSUANBIANXILINNA、LIUSUANLIANMEISU、TOUBAOLADINGJIQIZUHEYIZHIHUANGYERIBENTAOYESHANHUSHIGUANMIAOXIJUNWURANSHIYANJIEGUOBIAOMING,DANDUSHIYONG1ZHONGKANGSHENGSUSHI,KANGJUNJISHIGUANMIAOSHENGZHANG、SHENGGENQINGKUANGBURUZUHESHIYONGXIAOGUOHAO,FUJIAANBIANXILINNAYULIUSUANLIANMEISUGE50mg/L,DUIWURANJUNDEYIJUNLVWEI100%,ANBIANXILINNAYUTOUBAOLADINGKANGJUNZUHEXIAOGUOCIZHI,XIAOGUOHAOYULIUSUANLIANMEISUYUTOUBAOLADINGZUHE.JIANGBUTONGKANGSHENGSUJIARUJINXIANLIANPEIYANGJIZHONG,YIZHIXIJUNHEMEIJUNDESHENGZHANG,QUDELIANGHAOXIAOGUO,ZUIJIAKANGSHENGSUZUHEWEIZHIMEIJUNSU40U/ml、KANEIMEISU40U/ml、QINGDAMEISU200U/ml.

植物组培

捕鱼大作战  1.3 KANGSHENGSUZAIWURANMIAOQIANGJIUCHULIZHONGDEYINGYONG

  ZAIZHIWUZUZHIPEIYANGDEGUOCHENGZHONG,YIWURANDEWAIZHITIRENGKEYIYONGKANGSHENGSUJINXINGQIANGJIU,YIJIANSHAOSUNSHI.ZAIHONGZHANGZUPEIJIDAIPEIYANGJIZHONGJIARU5~20mg/LLIUSUANAMIKAXING,YOUXIAODIKONGZHILEQINGDUWURAN.ZAIMINIMEIGUIZUZHIKUAIFANTIXILIANXUJIDAIGUOCHENGZHONG,FAXIANPEIYANGJIZHONGJIARUANBIANQINGMEISU50~150mg/LYIZHILEWURANJUNSHENGZHANG,JIARULIUSUANDINGANKANEIMEISU25~100mg/LJINYIBUSHAMIELEWURANJUN.ZHENDUIYINBAIYANGZUZHIPEIYANGXIJUNWURANYANZHONGDEWENTI,CAIYONG400WANHUO600WANDANWEIDEQINGMEISUWUJUNSHUIRONGYECHULIXIJUNWURANMIAO,FENBIEJINPAO60minHUO40minKEYOUXIAOKONGZHIQIWURAN.ZAIJUHUAPEIYANGJIZHONGFUJIA50~150mg/LDEANBIANQINGMEISUHE50mg/LDELIUSUANDINGANKANEIMEISU,QIDAOYIZHIXIJUNSHENGZHANGDEZUOYONG,PEIYANGWUSHENGZHANGLIANGHAO.

  2 ZHONGYAOCAIZAIZHIWUZUPEIZHONGDEYINGYONG

  采用野生灵芝10g于1 000ml蒸馏水中煮2h的浸出液配制培养基,不仅能使香蕉正常生长,而且已感染细菌的材料经一段时间培养后,细菌污染消失.将苍术用于接种车间和培养车间的灭菌,结果发现苍术熏蒸的灭菌效果与紫外线照射及甲醛熏蒸无显著差异灭菌消毒效果维持的时间较长,而且对人体无刺激和毒副作用.王为研究中草药消毒剂的杀菌效果,选取大黄、艾叶等中草药在实验室制备了复方中草药消毒剂,并对其进行了定量杀菌试验、现场消毒试验等研究,发现以大黄、艾叶、丹皮、蛇床子、大青叶、苦参、五倍子组成的中草药消毒剂对金黄色葡萄球菌作用10min,杀灭率达99.9%,作用30min,可达100%,对大肠杆菌作用10min以上,杀灭率达100%,对枯草芽孢杆菌作用15min以上杀灭率达99.9%,表面现场消毒对细菌杀灭率达99.9%,培养基中有机物的存在对消毒效果有一定影响.既然中草药消毒剂可以杀菌,那么肯定能够推广应用于植物组培捕鱼大作战过程中的消毒灭菌.将传统的中药材应用于植物组织培养可谓是一大创举,其独特的优势决定了发展潜力的宽广.

捕鱼大作战相关的文章: 植物组培

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